1. Is diphenylamine (DPA) reaction DNA-specific (yes or no)? lf so, please expla

Question

1. Is diphenylamine (DPA) reaction DNA-specific (yes or no)? lf so, please explain why it does not react with RNAs, proteins, lipids and carbohydrates 2. On the standard curve of 2-deoxyadenylsine reacted with DPA, 0.33 mL of your DNA sample corresponded to 12.5 ug. What is the concentration of DNA in your sample? Show your calculations 3. Using the definition of "absorbance unit" described in the introduction of Chapter 8. Suppose you take 3 mL of a pure DNA solution and place it in a cuvette. You then add 3.75 mL of a buffer and mix the buffer and the DNA solution well. % Transmittance of this mixture at 260 nm was found to be 29. How much of the original DNA solution will contain 5 absorbance units? Show your calculations.

Explanation / Answer

DPA estimation method is a colorimetric method to quantify DNA wherein the deoxyribose sugar of DNA reacts with DPA in high acidic conditions to give a blue coloured complex. However, this reaction is non-specific as any 2-deoxyribose is capable of undergoing this reaction.

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