You set up the following gel in an attempt to construct a restriction map of the

ID: 307094 • Letter: Y

Question

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases Ban ll and Bg/ll. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... Lambda DNA cut with Bg/ll produces the following fragments: Describe the band corresponding to the 1807 bp fragment.

Explanation / Answer

The band corresponding to 1807 bp fragment is the 6th fragment from the top in lane number 1 and 6.The migration of a band depends on the length of the band i.e longer fragments travel slowly than smaller fragments in a gel.Also,from the fragments produced by Bg/II , we can say that the 6th fragment is the 1807 bp fragment.

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You set up the following gel in an attempt to construct a restriction map of the

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You set up the following gel in an attempt to construct a restriction map of the

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