On lab day, you will be given a set of plates that have three yeast strains on t

Question

On lab day, you will be given a set of plates that have three yeast strains on them: “a" and “alpha” parental haploid strains, and the diploid strain from the mating of “a” and “alpha”. The genotypes of the parent strains are:

“a” strain:   MATa hom3 TRP2 ade2

“alpha” strain:   MATalpha HOM3 trp2 ADE2

Predict the phenotype of your lab strains using the genotype information above. In the table below, write “growth” for the strains that will grow in the media and “NG” for the strains that will not grow.

Table 1. Phenotype predictions based on given genotypes.

Media

a

alpha

diploid

YPD

SC -threonine -methionine

SC -tryptophan

SC -adenine

SC -thr -met -trp

Minimal media

Media

a

alpha

diploid

YPD

SC -threonine -methionine

SC -tryptophan

SC -adenine

SC -thr -met -trp

Minimal media

Explanation / Answer

“a” strain:   MATa hom3 TRP2 ade2

x

“alpha” strain:   MATalpha HOM3 trp2 ADE2

Table 1. Phenotype predictions based on given genotypes.

Media

a

alpha

diploid

YPD

SC -threonine -methionine

SC -tryptophan

SC -adenine

SC -thr -met -trp

Minimal media

Media

a

alpha

diploid

YPD

MATa hom3 TRP2 ade2 MATalpha HOM3 trp2 ADE2 MATaMATalpha HOM3hom3 TRP2trp2 ADE2ade2

SC -threonine -methionine

MATalpha HOM3 trp2 ADE2 MATaMATalpha HOM3hom3 TRP2trp2 ADE2ade2

SC -tryptophan

MATa hom3 TRP2 ade2 MATaMATalpha HOM3hom3 TRP2trp2 ADE2ade2

SC -adenine

MATalpha HOM3 trp2 ADE2 MATaMATalpha HOM3hom3 TRP2trp2 ADE2ade2

SC -thr -met -trp

MATaMATalpha HOM3hom3 TRP2trp2 ADE2ade2

Minimal media

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