You will need to generate dropout plates to phenotype spore colonies for each po
ID: 308091 • Letter: Y
Question
You will need to generate dropout plates to phenotype spore colonies for each potential auxotrophy for the HOM3, TRP2, and ADE2 genes. Use the information in Table 2 to calculate the amount of supplements needed to make each dropout plate.
Table 2. Supplement calculation chart.
Supplements
L-Threonine
L-Methionine
L-Tryptophan
Adenine
How to make 100 ml of stock solution
4g/100 ml
1g/100 ml
1g/100 ml
0.5g/100 ml
Stock concentration
(in mg/ml)
Stock concentration
(in x)
200x
500x
500x
200x
Working concentration
(in mg/ml)
Working concentration
(in x)
Volume to add to petri plate in µl (volume of petri plate = 25 ml)
Supplements
L-Threonine
L-Methionine
L-Tryptophan
Adenine
How to make 100 ml of stock solution
4g/100 ml
1g/100 ml
1g/100 ml
0.5g/100 ml
Stock concentration
(in mg/ml)
Stock concentration
(in x)
200x
500x
500x
200x
Working concentration
(in mg/ml)
Working concentration
(in x)
Volume to add to petri plate in µl (volume of petri plate = 25 ml)
Explanation / Answer
Supplements
L-Threonine
L-Methionine
L-Tryptophan
Adenine
How to make 100 ml of stock solution
4g/100 ml
1g/100 ml
1g/100 ml
0.5g/100 ml
Stock concentration
(in mg/ml)
Stock concentration
(in x)
200x 40
8000mg/ml
500x 10
5000mg/ml
500x 10
5000mg/ml
200x 5
1000mg/ml
Working concentration
(in mg/ml)
Working concentration
(in x)
Volume to add to petri plate in µl (volume of petri plate = 25 ml)
Calculation:
*Stock concentration (L-Threonine)
=4000 mg/100ml =40 mg/ml =1x concentration
*for 200x concentration = 200 x 40 = 8000mg/ml
*Stock concentration (L-Methionine)
=1000 mg/100ml =10 mg/ml =1x concentration
*for 500x concentration = 500 x 10 = 5000mg/ml
*Stock concentration (L-Tryptophan)
=1000 mg/100ml =10 mg/ml =1x concentration
*for 500x concentration = 500 x 10 = 5000mg/ml
*Stock concentration (Adenine)
=500 mg/100ml =5 mg/ml =1x concentration
*for 200x concentration = 200 x 5 = 1000mg/ml
Supplements
L-Threonine
L-Methionine
L-Tryptophan
Adenine
How to make 100 ml of stock solution
4g/100 ml
1g/100 ml
1g/100 ml
0.5g/100 ml
Stock concentration
(in mg/ml)
40mg/ml 10mg/ml 10mg/ml 5mg/mlStock concentration
(in x)
200x 40
8000mg/ml
500x 10
5000mg/ml
500x 10
5000mg/ml
200x 5
1000mg/ml
Working concentration
(in mg/ml)
40 mg/ml 10mg/ml 10 mg/ml 5 mg/mlWorking concentration
(in x)
0.5 ml 0.2 ml 0.2 ml 0.5 mlVolume to add to petri plate in µl (volume of petri plate = 25 ml)
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