Circular dichroism (CD) is a technique used for determining the secondary struct

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Circular dichroism (CD) is a technique used for determining the secondary structure (presence of a-helix and beta-pleated sheet) of a protein. The authors took CD spectra of the protein at varying temperatures at various wavelengths. Then they plotted the results obtained at 220 nm. The results are an indication of the integrity of the secondary structure of the BN protein. A decrease from 100% indicates a loss of secondary structure integrity. The results are shown in Figure 7.2. What do these data tell you about the heat stability of the protein at various temperatures? How does heat stability of BN protein compare with other commonly known proteins? Figure 7.2: Single wavelength melting curve of the protease inhibitor BN from Brassica nigra (based on Ganov, eta!., 1997).

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Points which can be concluded from graph

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