You have been provided with a DNA nucleotide sequence that codes for a hypotheti

Question

You have been provided with a DNA nucleotide sequence that codes for a hypothetical protein. You will have to transcribe the code into mRNA and then translate that mRNA into the protein. Remember to pay attention to the start and stop codons so once the mRNA transcript is written out, scan from the beginning until you find the AUG start codon and divide the message up into triplet codons FROM THAT POINT. Then translate the amino acid sequence using the codon chart provided in the lesson.

DNA sequence

3' - AACAAACAATTTCCGTATAATCTTAATACATGGTGTAATCGTACCGGGACGGTT

TCGCGAGCATAGTGTACTTATATACAGTACTACGGACATGATAGTGGGTGTAAGA

ATACCCCCGGTGTACCTTGACACGGACTTGCAGGCATGCCTACCGTCG - 5'

Explanation / Answer

The DNA transcribes into the following mRNA sequence:

5' - UU GUU UGU UAA AGG CAU AUU AGA AUU AUG UAC CAC AUU AGC AUG GCC CUG CCA AAG CGC UCG UAU CAC AUG AAU AUA UGU CAU GAU GCC UGU ACU AUC ACC CAC AUU CUU AUG GGG GCC ACA UGG AAC UGU GCC UGA ACG UCC GUA CGG AUG GCA GC - 3'

AUG: Start Codon, codes for methionine (M)

UGA: Stop codon, doesn't code for any amino acid

The mRNA (5'->3' frame 3) gets translated and gives the following aminoacid sequence:

N-terminal - M Y H I S M A L P K R S Y H M N I C H D A C T I T H I L M G A T W N C A - C-terminal

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