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Which is NOT true A)DNA polymerase can add nucleotides onto a growing chain of D

ID: 314119 • Letter: W

Question

Which is NOT true

A)DNA polymerase can add nucleotides onto a growing chain of DNA at the speed of > 10 nucleotides per second.

B)One of the steps of the PCR reaction is carried out at near-boiling temperatures.

C)The PCR reaction has to be carried out at a low temperature to prevent DNA polymerase (the enzyme) from denaturing.

D)PCR can be used to amplify the DNA from a virus

A)DNA polymerase can add nucleotides onto a growing chain of DNA at the speed of > 10 nucleotides per second.

B)One of the steps of the PCR reaction is carried out at near-boiling temperatures.

C)The PCR reaction has to be carried out at a low temperature to prevent DNA polymerase (the enzyme) from denaturing.

D)PCR can be used to amplify the DNA from a virus

Explanation / Answer

C)The PCR reaction has to be carried out at a low temperature to prevent DNA polymerase (the enzyme) from denaturing.

Explanation: PCR uses thermostable DNA polymerase from thermus aquaticus, which is stable and functional at high temperatures

C)The PCR reaction has to be carried out at a low temperature to prevent DNA polymerase (the enzyme) from denaturing.

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