Annexin V is used to detect early-stage apoptosis because of its affinity for ph

Question

Annexin V is used to detect early-stage apoptosis because of its affinity for phosphatidyl-serine. When fluorescently labeled Annexin V is incubated with cells, the cells that are apoptotic are observed to fluoresce. What does this tell you about the condition of the membrane of the apoptotic cell? Why? For the polysaccharides in the left column, indicate all the descriptions in the right column that are appropriate. Provide a chemical explanation for each of your answers. Amylose Cellulose Dextran Glycogen Starch storage polysaccharide in yeasts and bacteria cannot be effectively digested by humans storage polysaccharide in humans

Explanation / Answer

18. Annexin 5 is a marker protein for apoptosis this protein have ability to bind phosphotidylserine when it is on outer membrane of plasma membrane. Externalization of phophotidyl serine is a intermediate stage of apoptosis by detecting this with the florescent dye we can confirm that which cell is apoptotic and which cell are normal in a population of cell.

19. Starch is storage polysaccharide in yeast and bacteria. it is made up of amylose and amylopectin amylose is a linear chain of thousand glucose molecule where as amylopectin is branched glucose molecule . they can be digested by breaking glycosidic bond.

Cellulose can not be digested by humans because human dont have enzyme to break beta acetal linkages which is important enzyme for breakdown of cellulose.

Glycogen is storage polysaccharide in humans. its is branched polymer of glucose molecule the glucose residues are linkes by -1,4-glycosidic bonds. Branches at about every tenth residue are created by -1,6-glycosidic bonds bond

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