Which of the following would you use for an Edman degradation reaction? a. Aceti

Question

Which of the following would you use for an Edman degradation reaction? a. Acetic anhydride b. Phenylisothiocyanate c. Urea d. 2-Mercaptoethanol e. CNBr (cyanogen bromide) The native structure of insulin involves mainly a. salt bridges b. hydrogen bonds c. hydrophobic (dispersion) forces d. disulfide bonds e. None of the above Cyanogen bromide (CNBr) is a reagent use in protein chemistry for the cleavage of polypeptide chains containing a. alanine b. methionine c. tyrosine d. Lysine e. Leucine. The amino acid sequence of a protein is NOT: a. a polypeptide b. encoded by the corresponding nucleotide sequence of DNA. c. a form of genetic information. d. read from N-terminal end to C-terminal end. e. constant for proteins with same function in different organisms. The information required for secondary and higher orders of protein structure is contained in a. hydrophobic interactions b. ionic bonds c. hydrogen bonds d. the primary structure e. the quat

Explanation / Answer

24. (b) Phenyl isothiocyanate

Phenyl isothiocyanate is one of the critical reagents used in Edman degradation

25. (d) disulfide bond

disulfide bond formation generates the native tertiary structure of insulin

26.(b) methionine

Cyanogen bromide hydrolyzes peptide bonds at the C-terminus of methionine residues.

27. (e)

28. (a) hydrophobic interactions (b) ionic bonds (c) Hydrogen bonds

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