Refer to Figure 1: Which of the following would be the best approach to eliminat

Question

Refer to Figure 1: Which of the following would be the best approach to eliminate unwanted cells?

a.Use antibodies to molecules such as Sca-1 to select mature cells

b.Use antibodies such as cKit to select stem cells

c.Use antibodies that will result in the elimination of the stem cells

d.Filter the suspension to remove the irregular shaped cells

In an experiment to enrich hematopoietic stems, positive selection was done to recover isolate HSC as shown in the cartoon in Figure 1. Unfortunately, after microscopic examination, it was found that there are some cells with different morphologies than what was expected. E ON Mo React with Negative selection Fl-antibodies against cells that to differentiation express mature (lineage) markers antigens a (8 Eo cells GE (N Differentiated React with Positive selection FI-antibodies for cells that against Sca-1 express stem cell and c-Kit markers (e.g., Sca-1 and c-Kit) S P Stem Progenitor cell Cells FIGURE 1

Explanation / Answer

to eliminate the unwanted morphological cells, d. Filter the suspension to remove the irregular shaped cells.

using antibodies may eliminate the desired cells as well. so filtration is the right approach.

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