In the process of cloning eukaryotic DNA into prokaryotic cells, the role of rev

Question

In the process of cloning eukaryotic DNA into prokaryotic cells, the role of reverse transcriptase is to make a. double-stranded eukaryotic cDNA from mature mRNA b. mature mRNA from precursor mRNA c. bacterial DNA from eukaryotic DNA d. mRNA from DNA 5 Which of the following strategies would help to improve ease of purification? a. Adding signal sequences that causes secretion into the culture medium b. Adding molecules that helps the protein to refold and stay in solution c. adding a sequence coding for an affinity handle Both a and c All of the above d. e. 6 Which of the following is an example of a protein recovery process? a. Large scale chromatography b. electroporation c. amplification d. fermentation hottell ae amnpci sens and ar td cin a. only cells that have not taken up the ampicillin resistant vector can grow b. only cells that have taken up the ampicillin resistant vector can grow C. all cells will grow d. ampicillin is inactivated

Explanation / Answer

Answers -

4. Double - standard eukaryotic cDNA from mature mRNA.

5. Adding a sequence coding for an affinity handle. and adding signal sequence that causes secretion into the culture medium.

6. Large scale chromatography

7. Only cells that have taken up the ampicillin resistant vector can grow.

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