Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how i

Question

Complementary DNA (cDNA) is a double-stranded molecule. In the laboratory, how is cDNA generated from a eukaryotic messenger RNA (mRNA)? Reverse transcriptase generates a single-stranded cDNA and then RNA polymerase synthesizes the complementary stran Reverse transcriptase generates a single-stranded cDNA and then uses that cDNA as a template to synthesize a complementary RNA strand. Reverse transcriptase generates a single-stranded cDNA and then DNA polymerase synthesizes the complementary stran Reverse transcriptase generates two single-stranded cDNAs from different mRNA molecules which hybridize to form double-stranded cDNA.

Explanation / Answer

(3) Reverse transcriptase generates a single stranded cDNA and then DNA polymerase synthesises the complimentary strand.

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