2. You are a new research scientist in a lab that studies microtubules. Your men

Question

2. You are a new research scientist in a lab that studies microtubules. Your mentor wants you to characterize a new cell line the she suspects expresses microtubules that behave strangely. You use CRISPR to tag the endogenous copy of p-tubulin ent protein (GFP) so that you can visualize it. By doing so you will be able to see how the microtubules in live cells respond to different conditions. Describe what would happen in the following scenarios: A. You add an aliquot from a bottle labelled "nocodazole" to your cells and yoiu see that the microtubules in the cells no longer depolymerize. You realize that there is no way you just added nocodazole to the cells. Explain in 1 sentence what made you realize that you didn't actually add nocodazole. What other drug did you most likely add?

Explanation / Answer

As shown above GTP is very important for polymerisation. If GTP will not be available, no polymerisation of depolymerisation will be possible in the cell and all dynamic processes in the cell as well as cell division will stop.

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