One of the main goals of this lab was to genetically transform some of the E. co

Question

One of the main goals of this lab was to genetically transform some of the E. coli bacteria. When you are observing the results in the next lab period, which two plates would you compare to determine if the bacteria had successfully taken up the plasmids?

Select one:

LB/amp +DNA ? LB/amp -DNA

LB/amp -DNA ? starter plate

LB/amp/ara +DNA ? LB/amp +DNA

LB -DNA ? LB/amp -DNA

Question 2

On which of the plates would you expect to find glowing bacteria colonies? Select all that apply.

Select one or more:

LB/amp -DNA

LB/amp +DNA

none of them

LB -DNA

LB/amp/ara +DNA

all of them

Question 3

On which of the plates would you expect to find lots and lots of colonies compared to the others? Select all that apply.

Select one or more:

LB/amp -DNA

LB/amp/ara +DNA

none of them

LB -DNA

all of them

LB/amp +DNA

Question 4

Another main goal of this lab was to observe the control of the arabinose operon, located in the plasmids given to some of the bacteria. When you are observing the results in the next lab period, which two plates would you compare to determine if the operon was switched on versus switched off?

Select one:

LB/amp/ara +DNA ? LB/amp +DNA

LB/amp +DNA ? LB/amp -DNA

LB/amp -DNA ? starter plate

LB -DNA ? LB/amp -DNA

Question 5

The "starter" plate contained the original E. coli that had not been genetically transformed. You used bacteria from that plate to create your four plates. Among those plates that you made, which one has bacteria most like the original E. coli?

Select one:

LB/amp/ara +DNA

LB/amp -DNA

LB -DNA

LB/amp +DNA

none of them

Question 6

Which of your four plates will contain genetically transformed bacteria? Select all that apply.

Select one or more:

LB/amp/ara +DNA

LB -DNA

LB/amp +DNA

LB/amp -DNA

none of them

all of them

Explanation / Answer

1)

We generally add a selectable marker to our plasmid that introduces into transformants a property. Here it is ampicillin resistance and hence the transformed e.coli will be able to grow on ampicillin resistant media (assuming that restriction site does not lie in the Ampr gene itself). Hence we would compare LB -DNA ? LB/amp -DNA

2)

The colonies will grow in LB/amp/ara +DNA, as this contains arabinose. This sugar when absorbed triggers production of Green Fluorescent protein which will lead to bacteria growing under UV light,

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