You are interested in finding bacteria that can grow on an unusual sugar, gulose

Question

You are interested in finding bacteria that can grow on an unusual sugar, gulose. You succeed, and are able to reconstitute the transporter in inverted (inside out) membrane vesicles – this allows you to vary the cytoplasmic components by simply adding them to the medium. You prepare the vesicles with equal concentrations of protons in and out of the vesicle, and varying concentrations of gulose inside the vesicle, with no gulose outside the vesicle. [hint: to answer these questions, it may help to draw what an inverted vesicle looks like, and where the individual molecules and reagents are located relative to the vesicle membrane at the beginning of the experiment.

You have five treatment groups, representing additions to the reaction solution, that is, to the exterior of the vesicles (= cytoplasm):

1.No addition

2. ATP

3. ATP plus gramicidin

4. NaOH (hint: how does this impact pH?)

5. NaOH plus gramicidin

The following are the two classes of results obtained, for varying concentrations of gulose loaded into the vesicle:

Part 1: For the five treatment groups, identify the predicted outcome (plot A or B) if the gulose transporter uses ATP, rather than proton motive force.

1._____

2._____

3._____

4._____

5._____

Part 2: For the five treatments, identify the predicted outcome (plot A or B) if the gulose transporter uses proton motive force, rather than ATP.

1._____

2._____

3._____

4._____

5._____

Explanation / Answer

Please find the answers below:

Answer 1:

Answer 2:

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