Have a BLAST Assignment Part 1: Find Yourself in DNA Due April 25 1. (2) Write t

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Have a BLAST Assignment Part 1: Find Yourself in DNA Due April 25 1. (2) Write the first 6 letters of your name skipping B, J.O,U,X, Z( first, middle and last names as needed) on the spaces provided and then use the codon chart to assign a mRNA coding sequence to the single letter amino acid code on the spaces provided. If you have a letter occuring twice or more in your name, use a second or third codon for that letter if it is available. e.g. for Dr.S: Name ?????s UCU ACU GAA GUU GAG AGC code Name CODE 2. (4) Go to BLAST (ttp:/blast.ncbi.nlm.nih.gov); in the BLAST screen, clic on NucleotideBLAST: In the large box, paste our type in your DNA coding strand (replacing U with T) sequence, finally elie on the BLAST button at the bottom. After waiting a bit, review the information presented, give first few species your spelling is most closely related to and the ratio: e.g. Spirometra erinaceieurpaei, a tapeworm 18/18 hits and Solanumn lycopersicum, a tomato with 17/18 hits for me Species 3.(4)Now go back to http://www.ncbi.nlm.nih.gov/BLAST/, clic Protein BLAST, put your 6 amino acid letters and BLAST it. View the resulting proteins that are the closest match to you name, noting that "predicted, putativeunnamed, hypothetical, and unknown" are predicted from genome sequences. Skip those and scan down until you find one that has a name e.g. coiled-coil domain containing protein form Enhydra lutris-sea otter 100% similarity for me. Report that name here: 4. (2) Why is there a difference, in my two search ooutcomes if not yours? Turn this in with the next assignment

Explanation / Answer

1. NAME: Joseph Jason

Letters corresponding to codons: S E P H A S N

mRNA code: UCU GAA CCU CAU GCU UCC AAU

2. Nucleotide sequence for BLAST : TCT GAA CCT CAT GCT TCC AAT

3. Amino acid sequence for BLAST:  S E P H A S N

Protein with 100% identity: protein SCAR1 isoform X1 [Cajanus cajan]

4. The difference may be due to the presence of exons or post translational modification.

Species Identity Larimichthys crocea 100% Amphiprion ocellaris 100% Helianthus annuus 100%

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