(2)Why is it important for the wells to be at the negative anode? (3)Draw the ba
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(2)Why is it important for the wells to be at the negative anode? (3)Draw the bands of DNA on your group’s gel and compare the fingerprints Procedure: 1. Prepare a 1% agarose gel in 1X TAE buffer. Heat flask in microwave at 30 second intervals until completely dissolved (should be absolutely clear). Allow the flask to cool for 5 minutes. Add Gel Red and pour into a gel casting apparatus as instructed by IA. 2. After the gel has set, remove the comb from the gel and rotate the gel in the electrophoresis chamber as directed by your IA. Make sure the wells are at the negative anode (black). (2) Why is this important? Cover the gel with approximately 400ml of 1X TAE 3. Make sure the battery supply is OFF. 4. Obtain 4 microcentrifuge tubes labeled: M (murderer), S1, S2, & S3 (suspects 1-3). Spin samples using tabletop centrifuge. Set a micropipette to 20ul. Load the contents of each tube (20ul) into separate wells of the gel as demonstrated by your IA. Be sure to load them in the 5. Place lids on chambers & plug the electrical leads into the power supply. Once all gels have been connected, turn voltage to 100V and start. Start time: Total electrophoresis time: 6. When the loading dye reaches the end of the gel, turn the power supply off and take the lid off of the electrophoresis chamber. 7. Carefully transfer the gel to the UV transillumintor. Be sure the protective cover of the UV transilluminator is CLOSED before turning on UV light. Failure to do so can lead to eye damage. (3) Draw the bands of DNA on your group's gel and compare the fingerprints. (4) Who was the murderer?Explanation / Answer
DNA is itself negative charged . So if you put gel electrophoresis Wells at positively charged cathod it will not move . So we put the wells are at the negatively charged anode so that after current flow DNA fragments moves towards the cathod and get separated by their molecular weight.
Question 3 and 4 is not able to answer because the main question or case is totally missing. If you are performing a DNA fingerprinting then I would suggest perform a restriction digestion of victim and as well as suspect and some DNA fragments isolated from victims body. Then run the gel whose band pattern will match with the band pattern of DNA found from victims body he/she will be murderer
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