Please help me with this question and give me the right answer. The roundworm Ca

Question

Please help me with this question and give me the right answer.

The roundworm Caenorhabditis elegans is a model organism that can be used to study the development of smooth muscle, also called nonstriated muscle. A C. elegans strain has been transformed with a lin-12::GFP reporter construct. The construct uses the lin-12 promoter to drive expression of a green fluorescent proteirn, GFP, which is expressed in several cell types, including the vulval muscle cells. The vulval muscles are smooth muscles that are critical for egg laying in C. elegans and are considered analogous to human smooth muscle, such as the intestinal muscles. The expression pattern of the construct in a wild-type C. elegans strain is shown below centered on the ventral region of the worm. How could you use lin-12:GFP worms to identify other genes involved in vulval muscle development? Knockout endogenous lin-12 and assay for functional vulval muscles Use microarray analysis to identify changes in gene expression of lin-12:GFP worms. Mutagenize lin-12::GFP worms and assay for abnormal or missing vulval muscles. Continued below.

Explanation / Answer

How could you use lin-12::GFP worms to identify other genes involved in vulval muscle development?

B) Use microarray analysis to identify changes in gene expression of lin-12::GFP worms.

For a gene identified above, referred to as a candidate gene, how could you use lin-12::GFP worms to determine if expression of the candidate gene is necessary and sufficient for vulval muscle development?

C) Generate a candidate gene knockout strain and assay for the presence of functional vulval muscles.

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