Consider: Your Bradford Assay acquired using a 50x dilution of your CFE sample g

Question

Consider: Your Bradford Assay acquired using a 50x dilution of your CFE sample gives a concentration of 0.37 mg/ml. What is the concentration of the undiluted CFE sample? 1. 2. Refer to your lab notebook to find the volume of CFE you prepared for your purification. Based on this value, what is the total protein content of your CFE sample? 3. Consider: Your Bradford Assay acquired using a 5x dilution of your D2 elution fraction gives a concentration of 0.19 mg/ml. What is the concentration of the undiluted D2 fraction? Assume this purified fraction contains only LDH, with a molecular weight of 36,500Da. What is the micro-molar (uM) concentration of LDH in this fraction? 4. 5. Which 3 amino acids possess intrinsic UV absorption properties that can be used to quantitate purified protein concentrations?

Explanation / Answer

Ans-1 the dilution is 50x it means 1 part of solvent and 49 parts of diluent. So the concentration before dilution can be calculated by multiplying dilution factor with final concentration.

= 0.37 ×50= 18.5 mg/ml.

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