10. A researcher performed the transduction experiment with a set ofb the follow

Question

10. A researcher performed the transduction experiment with a set ofb the following data: Phage Bacteriophage Bacteria titer MO1 Number of Transduction Percent Cells Titer (pfu/ml) (cfu/ml) (25 al phage+Transductants Frequency Transduced 100 ul bacteria) (25 al of phage 5.0x 1013s used) Alpha 5.7x 10 Beta 3.9 x 102 Gamma 8.0 x 10 135 45 178 3.0 x 10 From these data, determine the transduction frequency for each of the phage as well as the percent of cells that were transduced. Show your work and fill in your values in the data chart (9 pts)

Explanation / Answer

transduction frequency = no. of cells used / no. of phage used.

and

percent cell transduced = (no. of cells transduced / no. of cells used) * 100.

for

transduction frequency of alpha = 5*107/ 1.425*108 = 3.5*10-1 = 0.35.

and

percent cell transduced of alpha = (135 / 5*107 ) * 100 = 2.7 *10-4 %.

for

transduction frequency of beta = 4*107/ 9.75*1010 = 4.1*10-4

and

percent cell transduced of beta = (45 / 4*107 ) * 100 = 1.125 *10-4 %.

for

transduction frequency of gamma = 3*107/ 2*109 = 1.5*10-2

and

percent cell transduced of gamma = (178 / 3*107 ) * 100 = 5.9 *10-4 %.

MOI = virus used for infection / number of cells.

alpha = 5.7* 109 * 25* 10-3 / 5* 108 *0.1 = 2.85

beta = 3.9* 1012 * 25* 10-3 / 4* 108 *0.1 = 2.43 *103

gamma= 8* 1010 * 25* 10-3 / 3* 108 *0.1 = 66.66

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