Pre-lab questions 1. It is possible to transform an oorganism? Which Organism is

Question

Pre-lab questions 1. It is possible to transform an oorganism? Which Organism is most appropriate ? 2. How can you confirm that organism has been genetically transformed? 3. Predict the effecet of ampicilin on the transformed and non-transformed E. coli cells? 4. What is the function of CaClz on E coli cells? 5. Define competent cells 6. What is the function of heat shock during transformation? 7. On which of the plates would you expect to find bacteria most like the original non-transformed E. coli colonies you initially observed? Explain your predictions.

Explanation / Answer

yes, it is possible to transform an organism. single celled organisms are best recepients for genetic transformation as it contains only one cell which needs to take up the new gene to confirm that the organism has been genetically transformed,we need to look at the offsprings produced.if new trait is observed in the progeny then it confirms that genetic transformation has taken place transformed cells are resistant to the effect of ampicillin, hence it will survive whereas non transformed cells will die calcium chloride makes the E coli cells competant for the uptake of extraneous DNA by causing the overproduction of OmpC protein

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