11. Eight mutant bacteriophage strains cannot lyse a certain type of bacteria th
ID: 317543 • Letter: 1
Question
11. Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A "+" indicates that lysis occurred with coinfection. A "-" indicates that lysis did not occur.
(a) A cistron is defined by no complementation in the [Cis or Trans] Configuration.
(b) How many genes are mutated in this experiment? (Use a number not a word in the space) _______________
(c) Which of the following strains below are defective in the same gene as strain 7? Answer yes if the strain is defective or no if it is not.
1 2 3 4 5 6 7 8 1 - + + + + + - + 2 - + - + - + + 3 - + _ + + + 4 - + - + + 5 - + + + 6 - + + 7 - + 8 -Explanation / Answer
Answer:
a) Five strains are no complementation with either cis or trans configuration. From the table the follwing strains had no complementation 1&7, 2 &4, 2 & 6, 3 & 5, 4 & 6
b) 23 genes have mutated in this experiment which is shown by the lysis.
c) 1 & 7 are defective and others are not defective with respect to gene 7
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