For each of the three proteins calculate the number of tyrosines per 100 residue

Question

For each of the three proteins calculate the number of tyrosines per 100 residues and the number of tryptophans per 100 residues.

ignore second part of question.

1. For each of the three proteins calculate the number of tyrosines per 100 residues and the number of tryptophans per 100 residues. Does the intensity of bands correlate better with tyrosine content or tryptophan content? Chicken Egg White Lysozyme: KVFGRCELAAAM KKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSR WWCNDGRT PGSRNLCNIPCSALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKG TDVQ AWIRGCRL Bovine Serum Albumin DTHKSEIAHRFK DLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCE KSLHTLFGDELCKVASLRE TYGDMADCCEKKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDE FKADEKKFWNGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREK. ECADDRADLAKYlCDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDV CKNYQEAKDAFLGSFLYEYSRRHPEYA VSVLLRLAKEYEATLEEC (CAKDDPHACYSTVFDKLK HLVDEPQNLIKQN (CDQFEKLGEYGFQNALIVRYTRKVPQVSTP TLVEVSRSLGKVGTRCC TKP ESERMPCTEDYLSLIL NRLCVLHEKTPV SEKVTKCCTESLVNdRRPCFSALTPDETYVPKAFDE KL FTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACF AVE (GPKLVVSTQ TALA

Explanation / Answer

For first protein the tyrosine and tryptophan numer per 100 residue will be

toatal 3 tyrosine present in 130 residues so

3/130*100= 2.30 per 100 residue

toatal 6 tryptophan present in 130 residues so

6/130*100= 4.60  per 100 residue

For bovine serum Albumin the total no of amino acid is 584

Total tyrosine present is 18 so

18/584*100= 3.08 per 100 residue

only two tryptophan so

2/584*100=0.34 per 100 residues

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