3. The nucleotide sequences of the DNA molecules below are the same except for a

Question

3. The nucleotide sequences of the DNA molecules below are the same except for a single point mutation highlighted in bold

            DNA sequence                       5’-ATG ATC GGA TCG ATC CAT-3’

                                                3’-TAC TAG CCT AGC TAG GTA-5’

a)         Transcribe the sequence

(Hint: RNA polymerization is 5’ à3’, complementary to the 3’ à 5’ template)

Transcribed mRNA 5’ AUG AUC GGA UCG AUG CAU 3’

b) Amino acid sequence N-terminal Met - Ile - Gly - Ser - Met - His C-terminal

b)         Translate the sequence using the genetic code table.

Amino acid sequence N terminal met, ile, gly, ser, met, his c terminal

A mutation is defined as a change in the sequence of a DNA molecule, which can alter the structure or function of a protein. What are the new products of this mutation?

            Mutation I                   5’-ATGATCGGATTGATCCAT-3’

                                                3’-TACTAGCCTAACTAGGTA-5’

c) Transcribe:

d) Translate:

e) How is the mutated protein different?

Explanation / Answer

For sequence for  5’-ATG ATC GGA TCG ATC CAT-3’

DNA sequence 3'TAC TAG CCT AGC TAG GTA5'
m RNA sequecne    5'AUG GAU CGA UCC GAU CAU3'
Protein 1 Met Asp Arg Ser Asp His6

For mutation I

DNA sequence    3'TAC TAG CCT AAC TAG GTA5'
m RNA sequecne    5'AUG AUC GGA UUG AUC CAU3'
Protein       1 Met Ile Gly Leu Ile His 6

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