Goal: Dilution of food coloring with fewest pipette tips possible to minimize wa

Question

Goal: Dilution of food coloring with fewest pipette tips possible to minimize waste Exercise (Using forward pipetting method) Prepare the following micro tubes: Tube "A" 9 mu l of water Tube "B" 96 mu l of water Add 1 mu l of food coloring to Tube "A" Mix tube "A" and pipette 4 mu l of mixture of "A" into tube "B" What is the final dilution of tube "B" (x:y)? Exercise (Using reverse pipetting method) Prepare the following micro tubes: Tube "C" 98 mu l of water Tube "D" 975 mu l of water Add 2 mu l of food coloring to Tube "C" Mix tube "C" and pipette 25 mu l of mixture of "C" into tube "D" What is the final dilution of tube "D" (x:y)?

Explanation / Answer

Answer:

Tube A dilution:

Fold Dilution = (Stock volume)/(Stock volume + diluent)

Fold Dilution=1uL/(1uL + 9uL) =1/10

Tbe B dilution:

Fold Dilution=4/(4 + 96) =1/100

Answer: Final dilution = 1/10 x 1/100 = 1/1000

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