6. Which of the following does NOT correctly match a molecular technique with th

Question

6. Which of the following does NOT correctly match a molecular technique with the purpose for which it is used?

a. DNase I footprinting – identification of the portion of a specific DNA sequence to which a particular known protein binds

b. Chromatin immunoprecipitation (ChIP) – genome-wide identification of the DNA binding sites of a specific protein

c. EMSA (electrophoretic mobility shift assay) – detection of protein-protein binding activity of transcription factors upon protein purification

d. Linker scanning mutagenesis – identification of specific sequences that control transcription in a large regulatory region

Explanation / Answer

c. EMSA (electrophoretic mobility shift assay) – detection of protein-protein binding activity of transcription factors upon protein purification

Explanation- Electrophoretic mobility shift assay is a technique used to study whether protein or a mixture of proteins can bind with specific DNA or RNA sequence. Hence, it studies protein-DNA or protein-RNA interactions.

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