Suppose that branch migration occurs through gene 2. Let sequence \"b\" = GGATCG

Question

Suppose that branch migration occurs through gene 2. Let sequence "b" = GGATCGATC and "a" = GGTTCGATC. Note that the difference between the alleles is underlined.

Part B: What is the sequence on both strands of the two resulting chromatids? Use 5' & 3' to specify directionality, enter paired strands on separate lines so that the sequences align, enter "AND" on a line between chromatids. Example: 5'-GGATCGATC-3' 3'-CCTAGCTAG-5' AND 5'-GGTTCGATC-3' 3'-CCAAGCTAG-5'

Part C: Which strand would be used as the template for repair and how would it be identified?

Part D: How many possible results from repair exist, and how many are gene conversions?

Explanation / Answer

The holliday structure in the image uploaded is not visible. I will give you a general answer, please modify according to your image.

Imagine a holliday branch is formed somewhere near G_GATCGATC and "a" = G_GTTCGATC, in the dashed region. The the resulting strand after branch migration is.5' GGTTCGATC 3'/3' CCAAGCTAG3' AND 5'GGATCGATC 3'/ 3'CGTAGATAG5'.

The strand without methylation ( I.e the strand newsly formed ) is used as template.

The strand

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