1. Multiple Choices: (One and only one answer is correct-circle the cerrespondin
ID: 3308715 • Letter: 1
Question
1. Multiple Choices: (One and only one answer is correct-circle the cerresponding letter, 3 points each) 1. Which physical effect limits the resolution o an optical microscope A) Reflection B) Daffraction (C) Interference D) Scatering 2. Which of the following is true about the spherical aberation: (A) A spherical abemation occurs when a white light is usod B) Sample refractive index mismatch is the major cause of spherical aberration. (C) A spherical aberration occurs when different focusing occurs at twO perpendicular planes D) A spherical aberration comes from the ofF-axis light propagation. Nueical apeiture ofan objactive ls describes dhe A) ability of an optical system to show two close objects as separate B) ability of an optical system to magnify an image (C) ability of an optical syslam to cstimate the size ef an image D) size of an image. 4. Which of the following component is NOT the properties of light (A) Only the intensity of the light can only be detecned B) Phase shift cannot be measured. C) Light belongs to eectromagnetic wave D) Light is detected as particles . Dark field microscopy is: (A) A scanning microscopy lechnique B) A lechnsque based on coetrast agents. (C) Equivalent to spatial high-pass filtering at the Fourier plane. D) Equivalent to spatial low-pass fihering at the image plane. 6 Which of the following is the contrast mechanism for dark field micocopy (A) Absorption B) Birefringence (C) Optical path length difference. D) Scanering . Which of the following describes the main purpose of Kobler illurmination? (A) To minimine the spherical aberration Midter EX NAME B) To ebtain a uniform distribution of light intensity (C) To eblain a high-resolution image. D) To obtain a shorter depth of focus 8. good for achieving a deeper Which of the following properties are NOT penetration depth for tissue fluorescence imaging? (A) Near-infrased wavelength B) Matched refractive index between tissue and surrounding medium. C) Tissue scattering D) Ulta-bright fluorophores. . Which of the following properties is NOT from the intrinsic contrast in optical imaging? A) Scaered liglt from mitochondria B) Fluorescent marker attached to nucleic acid (C) Phase difference from a cell. D) Reflected light from a metal surfaceExplanation / Answer
1. The correct option is B) Diffraction.
Due to diffraction, the image spreads and the resolution reduces.
2. The correct option is D) A spherical aberation comes from the off-axis light propagation
Spherical aberration occurs due to the increased refraction of light rays when they strike a lens near its edge,
3. The correct option is A) ability of an optical system to show two close objects as separate.
The numerical aperture is a measure of its ability to gather light and resolve fine specimen detail at a fixed object distance.
4. The correct option is A) only the intensity of the light can only be detected.
For light, not only intensity, wavelength and phase can also be determined.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.