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1. In a calorimetric protein assay, 40 uL of a 0.40 mg/ml solution were mixed wi

ID: 33312 • Letter: 1

Question

1. In a calorimetric protein assay, 40 uL of a 0.40 mg/ml solution were mixed with 360 uL of water and 1.60 ml assay reagent. 1.00 ml was placeed in a cuvet. The abosrbance of 0.327. Calculate the sensitivity.

2. A protein assay gave the standard curve, A protein solution of known solution concentration was diluted 5-fold, then 30 uL were taken for assay. An absorbance of 0.200 (after blank subtraction) was observed. Calculate the protein concentration of the original undiluted solution.

3. Which amino acids affet the color yield for the Coomassie dye binding assay.

Explanation / Answer

1) The final concentration of the protein comes out to be 10

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