1- which staining technique provide the clearest presentation of bacterial morph

Question

1- which staining technique provide the clearest presentation of bacterial morphology?
2- list two acidic negative stins and four basic positives stains. indicate which has proton and which has hydroxide ions.
3- what specimen preparation best shown motility and why ?what stain technique implies motility.
4- why don't cells that have a acidic stains applied on them take on the color ?how do these cells appear under the microscope?
5-what is meant by counterstain?Give 2 examples?
6-which stans remain in a gram positive cell during a gram stain?why?
7-why do gram negative cells stain res/pink during the gram staining process, despite being treated with crystal violet and iodine?

Explanation / Answer

1. Grams' Stain
2.Acidic negative stains:Nigrosin and India Ink
Basic Positive stain: crystal violet, saffranine   
3. Hanging Drop Technique
5. an additional dye used in a microscopy specimen to produce a contrasting background or to make clearer the distinction between different kinds of tissue.
Example: Malachite green

6. Primary stain remains in Gram positive bacteria due to yhick peptidoglycan layer.
7.Gram negative bacteria have thin cell wall, due to which crystal violet washed and iodine washed off and retain the colour of saffranine.

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