Question 1 Assume that you wish to distinguish certain strains from a wild type

Question

Question 1

Assume that you wish to distinguish certain strains from a wild type strain. In the table write S for selective if the medium in each row can distinguish the strain indicated in each column from the wild type strain. If the medium cannot be used to distinguish the strain from the wild type strain write N for non-selective

Genotype

lac+leu-strs

lac-leu-strs

lac-leu+strs

lac+leu+strr

lac-leu-strr

Minimal

Minimal

+leucine

Minimal

- glucose

+lactose

Minimal

+streptomycin

Minimal

+leucine

+streptomycin

Minimal

-glucose

+lactose

+streptomycin

Minimal

+leucine

-glucose

+lactose

+streptomycin

Genotype

lac+leu-strs

lac-leu-strs

lac-leu+strs

lac+leu+strr

lac-leu-strr

Minimal

Minimal

+leucine

Minimal

- glucose

+lactose

Minimal

+streptomycin

Minimal

+leucine

+streptomycin

Minimal

-glucose

+lactose

+streptomycin

Minimal

+leucine

-glucose

+lactose

+streptomycin

Explanation / Answer

Genotype

lac+leu-strs

lac-leu-strs

lac-leu+strs

lac+leu+strr

lac-leu-strr

Minimal

Minimal

+leucine

Minimal

- glucose

+lactose

Minimal

+streptomycin

Minimal

+leucine

+streptomycin

Minimal

-glucose

+lactose

+streptomycin

Minimal

+leucine

-glucose

+lactose

+streptomycin

Genotype

lac+leu-strs

lac-leu-strs

lac-leu+strs

lac+leu+strr

lac-leu-strr

Minimal

S S S N S

Minimal

+leucine

N S S S N

Minimal

- glucose

+lactose

S S N S S

Minimal

+streptomycin

N N N S S

Minimal

+leucine

+streptomycin

N N N S S

Minimal

-glucose

+lactose

+streptomycin

S N N S S

Minimal

+leucine

-glucose

+lactose

+streptomycin

S S S S S

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