A generalized transducing phage is used to transduce an a ? ? b ? ? c ? ? d ? re
ID: 33608 • Letter: A
Question
A generalized transducing phage is used to transduce an a??b??c??d? recipient strain of E. coli with an a+?b+?c+?d+ donor. The recipient bacteria are cultured on various media selecting for specific donor genes. (Note that a? determines a requirement for A as a nutrient, and so forth.)
Selected gene
Unselected gene
a+
50% = d+
30% = b+
c+
50% = b+
a+ d+
10% = b+
a) Write a statement indicating the relationship between each gene and another.
(ie. __ must be very close to __ and somewhat close to __. etc
Selected gene
Unselected gene
a+
50% = d+
30% = b+
c+
50% = b+
a+ d+
10% = b+
Explanation / Answer
a) on the basis of given information about the percentage of selected and unselected gene the reationship between genes can be determined. In the recepient one or more donar marker is selected and then the presence of unselected marker is seen. Now by observing these percentages it is evident that a must be very close to d and somewhat close to b, and is distant from c. c is closer to b.
b) From the first part of the solution we have the following possibilities of order
a---d---b----c
c---b---d----a
Since, no particular gene is given for determining the order we select a for the same.
Therefore, the order of the genes in the complete donor chromosome will be:
a---d---b----c.
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