How do I solve this question? I don\'t just need the answer, I want to know how

ID: 34069 • Letter: H

Question

How do I solve this question? I don't just need the answer, I want to know how to solve it.

Based on the measurements in the table below, what is the concentration of a sample with an abosrbance of 0.52, to two decimal figures.

How do I solve this question? I don't just need the answer, I want to know how to solve it.

Based on the measurements in the table below, what is the concentration of a sample with an abosrbance of 0.52, to two decimal figures.

Concentration Absorbance 1.5 mg/ml 1.0 1.3 mg/ml 0.8 0.9 mg/ml 0.6 0.6 mg/ml 0.4 0.3 mg/ml 0.2 buffer only 0.0

I am renaming the table alphabehetically starting with a) to f).

a) being with concentration 1.5mg/ml and absorbance 1.0 and f) being buffer only with 0.0 absorbance

a) 1.5mg/ml concentrated protein has an absorbance of 1.0. So, absorbance of 0.52 would give protein concentration of

(0.52/1)* 1.5 = 0.78 mg/ml

b) (0.52/0.8)*1.3 = 0.845 mg/ml

c) (0.52/0.6)*0.9 = 0.78 mg/ml

d) (0.52/0.4)*0.6 = 0.78 mg/ml

e) (0.52/0.2)*0.3 = 0.78 mg/ml

f) buffer 0

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