5. Western blotting can be used to detect proteins that are translated from a pa

Question

5. Western blotting can be used to detect proteins that are translated from a particular mRNA. Rana Researcher is interested in the effects of mutation on the expression of a structural gene that encodes the Coco protein. Coco contains 572 amino acids. Its molecular mass is approximately 68,600 Da or 68.6 kDa. On the gel diagramed below mark the location of the Coco protein in the individual plants described. lane 41: A wild-type plant bane 42 A plant homozygous for a mutation that has removed the promoter Lane #3: A heterozygous plant where one of the alleles is wild-type and the other has a mutatic that introduced a stop codon at codon number 421. Lane 44: A plant homozygous for a mutation that changes codon 198 from a valine codon to a leucine codon.

Explanation / Answer

Answer

explanation :from the data and gel diagram we can come to the following genetic conclusions

lane 1: the wild type plant shows the presence of coco protein is susceptible to mutation

lane 2 : no proetin is seen in gel diagram which shows the plant homozygous is recessive for mutation.

lane 3 : heterozygous plant the protein has undergone partlial mutation hence one coco protein another protein having lower molecular weight has been formed

lane 4 plant homozygous for mutation forms one protein due to mutation at point 198 hence may have mol wt nearly 20kDa

Related Questions

Navigate

• Browse (All)
• Browse 5
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.