1) You need to prepare 30mls of Master Mix Cocktail composed of: 0.15M CAP buffe
ID: 34144 • Letter: 1
Question
1) You need to prepare 30mls of Master Mix Cocktail composed of:
0.15M CAP buffer
6mM NAD
0.15M sodium lactate
In the cabinet, you have the following ingredients: a) a stock solution of 1.5M CAP, (b) sodium lactate (solid; MW = 112 g), and (c) a vial of freshly reconstituted 10 mM NAD.
How much sodium lactate should you add to achieve the desired final concentration in your 30mls Cocktail? Round to two decimal places and include proper units.
2)
You need to prepare 30mls of Master Mix Cocktail composed of:
0.25M CAP buffer
10mM NAD
0.25M lactate
In the cabinet, you have the following ingredients: a) a stock solution of 1.5M CAP, (b) sodium lactate (solid; MW = 112 g), and (c) a vial of freshly reconstituted 50mM NAD.
How much sodium lactate should you use to get the desired final concentration in your 30mls Cocktail? Round to one decimal place and include proper units.
Explanation / Answer
1. Molecular weight of sodium lactate is 112 g.
Molarity = weight/ molecular weight * 1000/volume in mL
0.15 = weight/112 * 1000/30
weight = 0.504
weight of Soium lactate that should be added is approximately 0.5 grams.
2.
Molecular weight of sodium lactate is 112 g.
Molarity = weight/ molecular weight * 1000/volume in mL
0.25 = weight/112 * 1000/30
weight = 0.84 grams.
weight of Soium lactate that should be added is approximately 0.84 grams.
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