You are asked to purify a hexokinase. Discuss the parameters you would consider

Question

You are asked to purify a hexokinase. Discuss the parameters you would consider in sourcing and selecting such an enzyme, and briefly indicate how you would stabilize these proteins on isolation.

1. Which hexokinase would you think of purifying? Why? Think where would you find lots of this enzyme in a mammal? (You should be able to get this organ easily at the butcher’s from a pig or cow.)

2. will you find this enzyme in highest concentration in liver or muscle? Why?

3. once extracted, isolated, and purified how would you prove that you have indeed extracted a/this hexokinase?

Explanation / Answer

1) Hexokinase IV or glucokinase can be easily purified. It is abundant in liver, pancreas and small intestine

2) It is found in high concentrations in liver.

3) Glucokinase converts glucose into glucose-6-phosphate which can be tested for the additional phosphate group to confirm.

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