either side of the body? Activity 4: Using a Pregnancy Test to Demonstrate Antig

Question

either side of the body? Activity 4: Using a Pregnancy Test to Demonstrate Antigen-Antibody Reactions 1. In an ELISA a. the primary antibody binds to which molecule? b. the enzyme is attached to which molecule? c. the final step is the addition of which molecule? d. the secondary antibody binds to which molecule? 2. In a positive pregnancy test, which antigen is detected in urine? PART II. Putting It All Together . Review Questions nswer the following questions using your lecture notes, your textbook, and your lab notes. mpainkic ni . To which two organ systems does the thymus belong?

Explanation / Answer

1. In an ELISA,

a. The primary antibody binds to which molecule - Antigen
b. The enzyme is attached to which molecule -
If the ELISA id direct ELISA assay enzyme is attached to primary antibody congugate, if the ELISA is indirect or capture assay one enzyme is attached to secondary antibody conjugate.
c. The final step addition of which molecule: Substrate
d. The secondary antibody binds to which molecule - primary antibody

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