Name Section Part V: Hypertonic, Hypotonic, and Isotonic Solutions In this exerc
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Name Section Part V: Hypertonic, Hypotonic, and Isotonic Solutions In this exercise, water flows across the membrane to equilibrium. The isotonic solution contains the same salt (and same water) concentration as a living cell. The hypertonic solation contains more salt (and less water) and the hypotonic solution contains less salt (and more water) than a living cell. The effect of these solutions on living cells will be observed. Write a hypothesis predicting how a cell will be affected when immersed in a hypertonic solution Hypothesis: is placed in a bypey tonc sdlaten wats leaues tr celly ousi t to Shr wc The isotonic, hypertonic, and hypotonic solutions have already been prepared and are available at the supply area. The living cells used will be Elodea (a common aquarium plant) and red blood cells. 1. 2 3. The success of this exercise will depend largely upon two things: (1) the cleanliness of the slides, and (2) your care in not getting the three solutions mixed up after they are on the slides. Carefully clean three glass slides with a cleanser (found at the sink or supply area) and water 4. rinse, and dry 5. With a grease pencil or masking tape, label each slide hypertonic, isotonic, and solution. 6. For blood a. Obtain the bottle of sheep blood. b. Using a toothpick, place a very small drop of blood on each slide c. Add a drop or two of the appropriate solution (hypertonic, isotonic, hypotonic) to the previously labeled slides. d. Cover with coverslips, and observe under high power e. Using the high power objective of the microscope, observe areas where individual red blood cells can be clearly seen probably near the edge of the coverslip. f. Diagram the shape of one cell from each slide. For Elodea, obtain 3 young leaves. Then, a. Place one leaf on each of the appropriately labeled slides: b. Add 1-2 drops of appropriate solution (hypertonic, isotonic, hypotonic) to each slide: c. Cover with coverslip. d. After 5 minutes, observe the cells near the edge of the leaf under high power On the next page, diagram one cell from each slide. 7. 8. 56 Lab 6Explanation / Answer
Introduction: An experiment is performed to identify the effect of isotonic, hypertonic and hypotonic solutions on the living cells.
Hypothesis: The cells will be affected based on the concentration of the solutions surrounding the cells.
Isotonic solutions have the same salt concentration and hence no fluid will move in or out and hence the cells will be unchanged.
Hypertonic solutions have higher salt concentration then the living cells and hence the fluid will move out of the cell and the cells will shrink.
Hypotonic solutions have less amount of salt compared to the living cells which will allow the fluid from the surrounding to enter the cell and eventually the cell can rupture.
Observations:
Isotonic solutions have same salt concentration and hence the fluid will pass through the membranes with the same rates and hence when seen under the microscope the cells will retain their normal shape.
Hypertonic solutions have a higher salt concentration hence to achieve equilibrium the water will move out of the cell by osmosis and hence when seen under the microscope we can see that the cells have shrunk.
Hypotonic solutions have less amount of salt compared to the living cells, hence the fluid will move into the cell to achieve equilibrium by osmosis. When seen under the microscope we can see that the cells have swelled and they may also burst due to the pressure.
Conclusion: The experiment confirm our hypothesis that,
1)Isotonic solutions will not have a major effect in the size and shape of living cell.
2)Hypertonic solutions will decrease the size of the living cells.
3)Hypotonic solutions will increase the size of living cells or may cause rupture of the cells
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