total magnification = 100 X field diameter = 1.6 mm length of object = _________
ID: 3505124 • Letter: T
Question
total magnification = 100 X
field diameter = 1.6 mm
length of object = _________ um
1. Say you are observing an object in the low-power field. When you switch to high power, it is no longer in your field of view. Why might this occur ?
2. What should you do initially to prevent this from happening ?
3. Do the following factors increase or decrease as one moves to higher magnifiction with the microscope ? resolution ? working distance ? amount of light needed? depth of field ?
4. A student has the high-power lens in position and appears to be intently observing the specimen. The instructor, noting a working distance of about 1 cm, knows the student isn't actually seeing the specimen. How so ?
Explanation / Answer
1. Say you are observing an object in the low-power field. When you switch to high power, it is no longer in your field of view. Why might this occur ?
Answer:- When we switch from low power field to high power field, lens narrows the field view and the object is therefore no longer in our field as in low power field we have wider field of view which now under high power narrow field goes out of focus for adjusted object.
2. What should you do initially to prevent this from happening ?
Answer:- Initially to prevent this from happening we have to focus or center the object as much as possible before switching low power field to high power field.
3. Do the following factors increase or decrease as one moves to higher magnifiction with the microscope ? resolution ? working distance ? amount of light needed? depth of field ?
Answer:- resolution :- increase within limits
The ability to discriminate two close objects as separate is know as resolution and it increases within limits when one moves to higher magnifiction with the microscope .
working distance:- decrease
The working distance is the distance between the object and lens. The working distance decreases as one increase magnification.
amount of light needed:- increase
The amount of light needed decreases as magnification increases. There is a fixed amount of light per area, and when one increase the magnification of an area, he look at a smaller area.
depth of field:- decrease
The depth of field is a measure of the thickness of a plane of focus. As the magnification increases, the depth of field decreases.
4. A student has the high-power lens in position and appears to be intently observing the specimen. The instructor, noting a working distance of about 1 cm, knows the student isn't actually seeing the specimen. How so ?
Answer:- The working distance is the distance between the specific and objective lens and. For high power lens it should be around 0.5 mm and not about 1 cm to focus on object. Therefore student is not actually seeing the specimen.
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