5. The membrane potential difference ( or Vm) across the inner mitochondrial mem

Question

5. The membrane potential difference ( or Vm) across the inner mitochondrial membrane is -175 mV and the hydrogen concentration in the matrix is 10-7.8 M and the hydrogen concentration in the cytoplasm (intermembrane space) is 10-7.4 M. The temperature is 37C. a. What is the pH of the matrix and the cytoplasm b. calculate the free energy liberated when a mol of protons move down the electrochemical gradient c. if synthesis of ATP requires 14 kcal/mol how many protons would have to flow throught the F1F0 ATP synthase to synthesize a mol of ATP? d. convert .26V to the protonmotive force

Explanation / Answer

a. pH of the Matrix is 7.6 and the cytoplasm is 7.8

b. Delta G (Free energy) =n Delta E = -5.1 kcal/mol

c. 4 Protons have to flow throught the F1F0 ATP synthase complex.

d. The 0.26V will converted as protonmotive force is 1.02mA

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