In Lab1, you made dialysis bags and filled them with sucrose solution. Let’s ima

Question

In Lab1, you made dialysis bags and filled them with sucrose solution. Let’s imagine something different now: You make two dialysis bags and fill them with distilled water. You pat them dry, measure and record each one’s mass, and then place one in a beaker containing 0.5M sucrose solution and place the other one in a beaker containing 2.0M sucrose solution. After 15 minutes, you remove the bags, pat them dry, and measure and record mass again.
a.) Predict: Is the final weight (mass) greater or less compared to the initial weight (mass)?
b.) Do these dialysis bags represent cells that are in a hypertonic ECF or hypotonic ECF? Explain your answer.
c.) Predict: Which bag (one in 0.5M sucrose or one in 2.0M sucrose) should experience a larger change in mass? Why?
d.) How is the dialysis tubing membrane similar to a cell membrane?

Explanation / Answer

a)

Final weight (mass) will be less compared to the initial weight (mass).

b) Yes, this explains as the cells are present in hypertonic ECF. Like the exosmosis takes place when the cell is placed in hypertonic solution.

c) the bag placed in 2.0M sucrose solution will experience a larger decreased in mass, due to more concentration gradient and high osmotic potential of ECF.

d) Dialysis tubing is Semipermeable membrane like cell membrane because it allows only water through it.

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