3. A cell with an internal solute composition shown below (INSIDE) is put into a

Question

3. A cell with an internal solute composition shown below (INSIDE) is put into a solution with a different solute composition (OUTSIDE). Please describe the following: tonicity of the solution outside the cell, osmolarity of the solution outside the cell, any volume change that will occur in the cell. Additionally, explain whether the resting membrane potential is likely to be positive or negative, and why. (Hint: urea is one permeable solute)

INSIDE

OUTSIDE

25 mM NaCl

125 mM NaCl

2 mM urea

14 mM urea

5 mM cholesterol

20 mM cholesterol

150 mM KCl

5 mM KCl

25 mM proteins

5 mM proteins

5 mM MgCl2

4 mM MgCl2

2mM glucose

80 mM glucose

INSIDE

OUTSIDE

25 mM NaCl

125 mM NaCl

2 mM urea

14 mM urea

5 mM cholesterol

20 mM cholesterol

150 mM KCl

5 mM KCl

25 mM proteins

5 mM proteins

5 mM MgCl2

4 mM MgCl2

2mM glucose

80 mM glucose

Explanation / Answer

1. Tonicity of Solution outside thd cell

Hypotonic

2. Hyposmolar outside the cell

3. Volume change- Flow of solvant from inside to outside the cell

4. Resting membrane potential will be negative in this case

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