This is maternal (bold line) and paternal (dashed line) DNA strands of DNA that

Question

This is maternal (bold line) and paternal (dashed line) DNA strands of DNA that formed a TRIPLE holiday junction. I have a coupl questions about this, (please dont just answer one of them):
1. In how many ways can this be resolved with or without recommendation occuring?
2. How many ways can this be resolved leading to recombination? And where are those places?
3. If the 5’ was given what primes are the remaining ends marked A-G ??
I understand single & double holiday junctions. But this triple holiday junction is confusing. A. Ca

Explanation / Answer

A holiday junction is a branched nucleic acid structure that contains 4 double stranded arms joined together.

branch migration is a fundamental step in the process of DNA recombination that determines the location,extent,and of the exchange between the recombining duplexus.a recombining duplexes contain intermolecular triplexes.triple stranded DNA with 3 oligonucleotides wind around each other forming a triple helix.in this in the above example F5 is the maternal and EB is the paternal bond.

the third bond can be of various forms

1.intermolecular formation---this triplex refers to a combination between a duplex and a different strand of DNA.this third strand can be of the neighbouring chromosome or a triplex forming oligonucleotide.

2.intramolecular formation----this triplex can be formed from a homopurine and a homopyrimidine strands.these are of two types--H-DNA & H*-DNA.

so in the above given example this combination can be intermolecular or intramolecular .homopurine or homopyridine where binding of homopurine and purine strands of the duplex in an antiparallel fashion takes place.

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