8. Calculate the magnification of fields 1 and 3, and the field diameter of 2. (

Question

8. Calculate the magnification of fields 1 and 3, and the field diameter of 2. (Hint: Use your ruler.) Note that the numbers for the field diameters below are too large to represent the typical compound microscope lens system, but the relationships depicted are accurate. 5 mm 0.5 mm 2-O- 100 X 9. Say you are observing an object in the low-power field. When you switch to high power, it is no longer in your field of view. t this occur? Decause he zoom leavey e vieu What should you do initially to prevent this from happening? do 10. Do the following factors increase or decrease as one move? to higher magnifications with the microscope? resolution: amount of light needed: working distance: depth of field: 11. A student has the high-power lens in position and appears to be intently observing the specimen. The instructor, noting a working distance of about 1 cm, knows the student isn't How so? Powe lets realeworking dbs 12. Describe the proper procedure for preparing a wet mount. 13. Indicate the probable cause of the following sijuations during use oa m croscope. a. Only half of the field is illuminated: Slide aud b. The visible field does not change as the mechanical stage is moved:

Explanation / Answer

8.5mm 2.5mm 0.5mm

50x 100x 500x

9.

A.the field decreases proportionally as magnification increases. Therefore unless the object is centred at low power , it might be outside the higher the higher power filed .

B.center the object that you wish to view.

10.resolution.increases to a point

Increases

Working distance decreases

Depth of field decreases

11.the working distance for the h.p lens is closer to 1mm.

12.place the specimen on the slide with a dropper. Place a drop of water or saline on the field .Mix specimen into the drop using a toothpick .if staining add a drop of stain and mix with toothpick.place the cover slip carefully to avoid air getting in .

13.A.The lens is not correctly into place .

B.the slide is not correctly positioned in the clamp on the mechanical stage and does not move when the mechanical stage moves.

Thanks

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