. Histone deacetylases (HDACs) are metalloenzymes that are very important for ge
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Question
. Histone deacetylases (HDACs) are metalloenzymes that are very important for gene regulation and have been identified with cancer proliferation. Immobilizing HDAC enzymes on solid support for screening would be a good strategy for drug discovery. a. DNA microarrays are emerging as a valuable tool to immobilize cells, enzymes and other biomolecules on solid supports. Devise a strategy to link a single DNA strand on a single position on an HDAC enzyme without inhibiting its function or the ability of the DNA strand to hybridize with its complementary strand on the solid support. Make sure to show all appropriate structures, linkages and strategies of enzyme/DNA modifications.Explanation / Answer
a)instead of microarray solid support can be used like different protein base like avidin or azide coated plate. it can be preformed like firstly HDACs are incorporated on solid surface like microarray chip with any taq either fluorescent one than the specificDNA is incorporated on histone protein via negative _ positive interaction without effecting the function .
b) ELISA is a different way to devise the methodology. Where firstly an antibody is incorporated than anti antibody is used which bind specifically with the dna simply without effecting it's function.
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