The following DNA sequence represents a eukaryotic gene. Indicate which is the t

Question

The following DNA sequence represents a eukaryotic gene. Indicate which is the template and which is the coding strand, determine where transcription begins (assume it ends at the end of the sequence presented), and write out the nucleotide sequence of the initial transcript. You need to process the initial transcript to make an mRNA (there is an intron in the gene; using the information about splicing locate and remove the intron) and write out the sequence of the completely processed mRNA. Finally, translate the mRNA and write out the amino acid sequence of the encoded polypeptide.5’-ACCGGCCTCTCTCTCACACACAGTACGGCGTTTATTGGGACTTCAAATCATATGGCCATTACAAGCCCTGCAGAAAGGGAAGGCGAAGCTCAGGATAAGTA...3’-TGGCCGGAGAGAGAGTGTGTGTCATGCCGCAAATAACCCTGAAGTTTAGTATACCGGTAATGTTCGGGACGTCTTTCCCTTCCGCTTCGAGTCCTATTCAT......CAGGTGACACTCTACTCACCGGATAAGTATTGTCATCTCCCAAGGGGGTGAATATCCGCTGGTCATCCGCTTTATATCGCCTGATTAACGCAGTCCTACACAAT-3’...GTCCACTGTGAGATGAGTGGCCTATTCATAACAGTAGAGGGTTCCCCCACTTATAGGCGACCAGTAGGCGAAATATAGCGGACTAATTGCGTCAGGATGTGTTA-5’

Explanation / Answer

1) The coding strand is the segment within double stranded DNA that runs from 5' to 3' and template strand which runs from 3' to 5'. so from given DNA sequence the answer is as follows:

CODING STRAND: a)ACCGGCCTCTCTCTCACACACAGTACGGCGTTTATTGGGACTTCAAATCATATGGCCATTACAAGCCCTGCAGAAAGGGAAGGCGAAGCTCAGGATAAGTA...3’  AND

b)TGGCCGGAGAGAGAGTGTGTGTCATGCCGCAAATAACCCTGAAGTTTAGTATACCGGTAATGTTCGGGACGTCTTTCCCTTCCGCTTCGAGTCCTATTCAT......CAGGTGACACTCTACTCACCGGATAAGTATTGTCATCTCCCAAGGGGGTGAATATCCGCTGGTCATCCGCTTTATATCGCCTGATTAACGCAGTCCTACACAAT-3’

TEMPLATE STRAND:GTCCACTGTGAGATGAGTGGCCTATTCATAACAGTAGAGGGTTCCCCCACTTATAGGCGACCAGTAGGCGAAATATAGCGGACTAATTGCGTCAGGATGTGTTA-5’

2) determine where transcription begins: transcription in eukaryotes begins with an enzyme called RNA POLYMERASE  attaches to the DNA template at a specific area this area known as promoter region often includes a specialized nucleotide sequence TATAAA which is also called the TATA BOX.

3) the sequence of the completely processed mRNA: CCGCUGGUCAUCCGCUUUAUAUCGCCUGAUUAACGCAGUCCUACACAAU-5'

4) translate the mRNA and write out the amino acid sequence of the encoded polypeptide.5 : ans is

CCG Pr

CUG Leu

GUC val

AUG met(chain initiation codon)

CGG arg

UUU phe

AUG met(chain initiation codon)

UCG ser

CCU pro

GUU val

UUU phe

CGC arg

UGC cys

CCU pro

UGU cyc

GUU val

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