Rot analysis (analysis of hybridization kinetics of cDNA-mRNA) has been used to
ID: 35373 • Letter: R
Question
Rot analysis (analysis of hybridization kinetics of cDNA-mRNA) has been used to determine the complexity of an mRNA population. Data presented below is from a Rot analysis of an mRNA prepared from the liver of a male rainbow trout treated with estrogen. (A). What conclusion can you draw from this hybridization kinetics? (B). Can you outline an experiment that may help you to determine how many species of mRNAs may be present in component (I) indicated in the figure?
Rot analysis (analysis of hybridization kinetics of cDNA-mRNA) has been used to determine the complexity of an mRNA population. Data presented below is from a Rot analysis of an mRNA prepared from the liver of a male rainbow trout treated with estrogen. (A). What conclusion can you draw from this hybridization kinetics? (B). Can you outline an experiment that may help you to determine how many species of mRNAs may be present in component (I) indicated in the figure?Explanation / Answer
(A) This graph is showig hybridisation between excess mRNA and cDNA. It identifies several components in the liver cells of a male rainbow trout treated with estrogen.
(B)
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