Hint : The methylation of DNA enables the cell\\\'s machinery to distinguish bet
ID: 35923 • Letter: H
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Hint: The methylation of DNA enables the cell's machinery to distinguish between the template strand and the newly synthesized strand. Consider which sequence is methylated by Dam methylase.
Note: Not all the terms will be used, and two terms will be used more than once.
The steps of mismatch repair in E. coil replication are given below. Note that in this case the mismatch is on the 3' side of the partially methylated DNA sequence. Complete the sentences by moving the correct term to each blank. Not all the terms will be used, and two terms will be used more than once. The template strand is methylated at 5'- -3' sequences; for a short time, the new, complementary strand is not. A DNA duplex that has only one methylated strand is . forms a complex with MutS at the mismatched base pair. A loop is formed as (bound to the partially methylated sequence) and the -MutS complex interact, and cuts the nonmethylated strand. A(n) degrades the nonmethylated DNA strand between the cleavage site and just beyond the mismatch site. DNA fills in the gap. DNA joins the nick.Explanation / Answer
The templated strand is methylated at 5'-GATC-3'sequences;for a short time,the new ,complementary strand is not.
A DNA duplex that has only one methylated strand is hemimethylated.
MutL forms a complex with MutS at mismatched base pair.
A loop is formed as Endonuclease (bound to the partially methylated sequence)and the MutL-MutS complex interact,and Endonuclease cuts he nonmethylated strand.
An Exonuclease degrades the nonmethylated DNA strand between the cleavage site and just beyond the mismatch site.
DNA polymerase 3 fills in the gap.
DNA Ligase joins the nick.
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