The following data were obtained from partial cleavage and analysis of a pentade

Question

The following data were obtained from partial cleavage and analysis of a pentadecapeptide: CNBr digestion peptides (not n order): CNBrI: Ala, Gly, Leu, Lys, Met, Pro CNBr2: Ala, Arg, Asp, Cys, GIn, Gly CNBr3: Met, Phe, Trp Trypsin digestion peptides (not in order): TI: Ala, Cys, GIn T2: Ala, Gly, Lys, Met, Phe, Trp T3: Arg, Asp, Gly, Leu, Met, Pro N-terminus: Phe (for the pentadecapeptide) Gly (for CNBrI) Asp (for CNBr2) Cys (for TI) Leu (for T3) C-terminus: GIn Determine the sequence of the pentadecapeptide. Use three-letter abbreviations in your answer. Use dashes (?) between each abbreviation.

Explanation / Answer

As we know, CNBr will cleave after Met.

So we can say that the sequence will be

From C3   Phe___Met

From C1   Gly__ __ __ __ Met

From C2    Asp __ __ __ __ Gln

As Trypsin digests after Arg / Lys

For T2 Phe __ __ __ __ Lys

For T3 Leu __ __ __ __ Arg

For T1 Cys- Ala -Gln

The amino acid at C-terminus is Gln

So using the above information we can predict the sequence of the pentadecapaptide

Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln

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