You decide to use cotransduction to determine the order of the leuB and leuD loc

Question

You decide to use cotransduction to determine the order of the leuB and leuD loci. You use bacteriophage to transduce DNA from an ala3 +leuB +leuD +donor strain into an ala3 leuBleuD recipient strain and select for 10,000 ala3 +transductants. You determine the genotypes of these transductants at the leuB and leuD loci and count the number with each genotype. The following table shows your results:

Using these data, determine the order of leuA, leuB, and leuD. Place the three loci on the map by dragging the correct label to the appropriate location. (Note: The map is not drawn to scale.)

You decide to use cotransduction to determine the order of the leuB and leuD loci. You use bacteriophage to transduce DNA from an ala3 +leuB +leuD +donor strain into an ala3 leuB leuD recipient strain and select for 10,000 ala3 +transductants. You determine the genotypes of these transductants at the leuB and leuD loci and count the number with each genotype. The following table shows your results: Using these data, determine the order of leuA, leuB, and leuD. Place the three loci on the map by dragging the correct label to the appropriate location. (Note: The map is not drawn to scale.)

Explanation / Answer

The answer is D, B, and A. The least amount shown will have the most crossovers. In order to do so, ala, D, and B have to be close together.

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